Clean, green, renewable biofuels from bacteria

US Department of Energy researchers from the Joint BioEnergy Institute (JBEI) reported in a recent paper that they have engineered the first bacterial strain that can digest the biomass of non-food crops and synthesize the sugars into all three forms of transportation fuel - gasoline, diesel and jet biofuels.
Biofuels made from non-food crops and agricultural waste, such as switchgrass, are thought to be the best pathway towards renewable transportation fuels. Unlike ethanol, which in the US is produced from corn starch, these advanced biofuels can replace gasoline on a gallon-for-gallon basis, and they can be used in today’s engines and infrastructures. But bringing the cost of producing these fuels down so that they are economically competitive has served as the biggest roadblock to date.
Part of the difficulty in using plant biomass as a fuel source lies in extracting the complex sugars - cellulose and hemicellulose - which are embedded in a tough woody material called lignin. Once extracted, these complex sugars must then be converted (or hydrolyzed) into simple sugars before they can be synthesized into fuels.
The Proceedings of the National Academy of Sciences (PNAS) paper describes how the team genetically modified Escherichia coli to express several key enzymes that enabled the bacteria to grow on switchgrass. These 'cellulolytic' and 'hemicellulolytic' strains of E. coli, were further engineered with three metabolic pathways that enabled the bacteria to produce fuel substitute or precursor molecules suitable for gasoline, diesel and jet engines.
“This work shows that we can reduce one of the most expensive parts of the biofuel production process, the addition of enzymes to depolymerize cellulose and hemicellulose into fermentable sugars,” says Jay Keasling, CEO of JBEI and leader of this research. “This will enable us to reduce fuel production costs by consolidating two steps – depolymerizing cellulose and hemicellulose into sugars, and fermenting the sugars into fuels – into a single step or one pot operation.”
One of the keys to the success of the JBEI team was the use of an ionic liquid (i.e. molten salt) to pre-treat the biomass before introducing the modified E. coli.
Gregory Bokinsky, a post-doctoral researcher with JBEI’s synthetic biology group and lead author of the PNAS paper, commented; “The magic is in the ionic liquid pre-treatment. If properly optimized, I suspect you could use ionic liquid pre-treatment on any plant biomass and make it readily digestible by microbes. For us it was the combination of biomass from the ionic liquid pretreatment with the engineered E. coli that enabled our success.”
The researchers believe that the techniques they've developed should be easily adapted to other microbes. This would open the door to the production of advanced biofuels from lignocellulosic feedstocks that are ecologically and economically appropriate to grow and harvest anywhere in the world.



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